Analysis Note

ControlEGF-treated and untreated A431 cell lysate.

Application

This Anti-mTOR Antibody is validated for use in WB for the detection of phospho-mTOR (Ser2448).

Research Sub CategoryPI3K, Akt, & mTOR SignalingGlucose/Glycogen MetabolismInsulin/Energy SignalingAdhesion (CAMs)

Immunogen

Amino acids encompassing and including phosphorylated Ser2448 of human mTOR

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified rabbit polyclonal IgG in storage buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by western blot on EGF-treated and untreated A431 cell lysate.

Western Blot Analysis:
This antibody detected mTOR phosphorylated on Ser2448 in EGF-treated and untreated A431 cell lysates.

Specificity

Predicted to cross react with rat, mouse, sheep & Rhesus Macaque based on 100% sequence homology.

Recognizes mTOR when phosphorylated on Ser2448

Target description

~289 kDa observed.
An uncharacterized band may be obsereved at ~59 kDa